Korean Journal of Medicinal Crop Science (한국약용작물학회지)
- Volume 1 Issue 2
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- Pages.184-190
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- 1993
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- 1225-9306(pISSN)
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- 2288-0186(eISSN)
Callus Induction and Somatic Embryogenesis in Suspension Culture of Rehmannia glutinosa
지황의 캘러스 유도와 현탁배양에서 체세포배 발생
- Chae, Young-Am (Agronomy Department and Research Center for New Biomaterials in Agriculture of Seoul National University) ;
- Park, Sang-Un (Agronomy Department and Research Center for New Biomaterials in Agriculture of Seoul National University)
- Published : 1993.09.22
Abstract
This study was carried out to investigate the appropriate medium and constitutionof growth regulators for somatic embryogenesis for development of rapid mass propagation system via somatic embrygenesis in Rehmennia glutinosa. Embryogenic callus formation from leaf explant was more effective when 4mg / l BA with 0.5mg / l NAA than that of treated with only auxins or cytokinins. LS medium was suitable for embryogenic callus formation. LS medium with 4mg / l BA with 0.5mg / l NAA was effective for the maintenance and proliferation of embryogenic callus. In suspension culture, addition of 1mg / l BA to LS medium was proper for somatic embryogenesis. The highest rate of shoot developement form cotyledon stage embryo was obtained in 1/2 LS medium and plantlet survived by 75% after transplanted to the soil. after 4 weeks.
1. 지황의 잎 조직에서 캘러스 유도는 옥신류와 사이토키닌류의 단독처리보다는 BA와 NAA를 혼합처리하는 경우에 캘러스 유도가 잘 되었다. 2. 옥신류의 embryogenic 캘러스 유도는 5% 정도로 극히 낮았으나 사이토키닌류를 처리한 경우는
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