Korean Journal of Pharmacognosy (생약학회지)

The Korean Society of Pharmacognosy (한국생약학회)
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Compositional Analysis of Polysaccharide from Sanguisorba officinalis and Its Anticoagulant Activity

지유로부터 분리한 다당류의 분석과 항응고작용

  • Kim, Yeong-Shik (Natural Products Research Institute, Seoul National University) ;
  • Roh, Ji-Eun (College of Pharmacy, Dongduck Women's University) ;
  • Ann, Hyung-Soo (College of Pharmacy, Dongduck Women's University)
  • 김영식 (서울대학교 천연물과학연구소) ;
  • 노지은 (동덕여자대학교 약학대학) ;
  • 안형수 (동덕여자대학교 약학대학)
  • Published : 1993.06.30
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Abstract

Polysaccharide from Sanguisorba officinalis was separated and fractionated using DEAE-Sephadex ion-exchange chromatography and Sephacry HR-200 gel filtration chromatography. One of the fraction(Fr. II) was sulfated and its anticoagulant activity was tested in vitro. Sulfation could increase the clotting time 50 times compared to unsulfated one. Fr. II was hydrolyzed and its composition was analyzed by conjugation with 7-amino-1, 3-naphthalene disulfonic acid using HPLC and electrophoresis. Arabinose and galactose were mainly composed at the ratio of 4 : 1. In addition, xylose and rhamnose were also found.

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