The Korean Journal of Physiology

The Korean Physiological Society (대한생리학회)
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Role of $Na^+\;-K^+$ Pump on Endothelium-dependent Relaxation

  • Sung, Sang-Hyun (Department of Thoracic surgery Kangnam General Hospital) ;
  • Roh, Joon-Ryang (Department of Thoracic surgery College of Medicine Seoul National University) ;
  • Park, Tae-Sic (Department of Obstetrics and Gynecology College of Medicine Seoul National University) ;
  • Suh, Suk-Hyo (Department of Physiology College of Medicine Seoul National University and Dankuk University) ;
  • Hwang, Sang-Ik (Department of Thoracic surgery College of Medicine Seoul National University) ;
  • Kim, Ki-Whan (Department of Thoracic surgery College of Medicine Seoul National University)
  • Published : 1993.12.01
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Abstract

To study the underlying mechanism through which the endothelium-dependent relaxation is inhibited by blocking the $Na^+\;-K^+$ pump, the effects of $Na^+\;-K^+$ pump blockade on the release of EDRF and its relaxing activity were examined, using organ bath study, bioassay technique, and cGMP measurement. Endothelium-dependent relaxation was attenuated by blocking the $Na^+\;-K^+$ pump in the vascular ring with intact endothelium. In bioassay experiment EDRF release was decreased with the blockade of the $Na^+\;-K^+$ pump in the EDRF donor strip. Endothelium-dependent increase of cGMP level was suppressed by inhibiting the $Na^+\;-K^+$ pump in the test strips. The magnitude of relaxation of test strip which was induced by the perfusate that had passed through the EDRF donor strip was decreased with the blockade of the $Na^+\;-K^+$ pump in the test strip. Therefore, it could be suggested that the attenuation of endothelium-dependent relaxation caused by inhibiting $Na^+\;-K^+$ pump activity is due to both the decreased release of EDRF from endothelial cells and the decreased sensitivity of the smooth muscle cells to EDRF.

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