The Korean Journal of Physiology
- Volume 27 Issue 2
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- Pages.151-162
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- 1993
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- 0300-4015(pISSN)
Effect of Cyclic GMP on the Calcium Current in Rabbit Ventricular Myocytes
- Han, Jin (Department of Physiology and Biophysics, College of Medicine, Inje University) ;
- Leem, Chae-Hun (Department of Physiology and Biophysics, College of Medicine, Seoul National University) ;
- Ahn, Chul-Soo (Department of Physiology and Biophysics, College of Medicine, Dankuk University) ;
- So, In-Suk (Department of Physiology and Biophysics, College of Medicine, Seoul National University) ;
- Kim, Eui-Yong (Department of Physiology and Biophysics, College of Medicine, Inje University) ;
- Ho, Won-Kyung (Department of Physiology and Biophysics, College of Medicine, Seoul National University) ;
- Earm, Yung-E (Department of Physiology and Biophysics, College of Medicine, Seoul National University)
- Published : 1993.12.01
Abstract
In order to investigate the effect of intracellular cyclic GMP on calcium current the whole-cell patch clamp technique with internal perfusion method was used in isolated ventricular myocytes of the rabbit. Cyclic GMP, 8-bromo-cyclic GMP, cyclic AMP, isoprenaline and forskolin were perfused into cells and their effects on calcium current were analysed by applying depolarizing step pulses of + 10 mV in amplitude far 300 msec from holding potential of - 40 mV. Not only cyclic AMP
Keywords
- Ventricular myocytes;
- Whole-cell patch clamp;
- Calcium current;
- Cyclic GMP;
- 8-Bromo-cy-clic GMP;
- Cyclic GMP-dependent protein kinase