The Journal of Natural Sciences (자연과학논문집)

Research Institute of Natural Sciences (배재대학교 자연과학연구소)
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Regulation of $\beta$-Galactosidase Biosynthesis in Alkalophilic, Thermophilic Bacillus sp. TA-11

호알칼리성, 고온성 Bacillus sp. TA-11의 $\beta$-galactosidase의 생합성 조절

  • Lee, Jong-Su (Dept. of Genetic Engineering, Pai Chai University, Taejon, Korea 302-735) ;
  • Lee, Hyang-Sook (Dept. of Biology, Graduate School of Pai Chai University, Taejon, Korea 302-735)
  • 이종수 (배재대학교 유전공학과) ;
  • 이향숙 (배재대학교 대학원 생물학과)
  • Published : 1992.09.30
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Abstract

Regulation of $\beta$-galactosidase biosynthesis was studied with alkalophilic, thermophilic Bacillus sp. TA-11. Biosynthesis of the enzyme was effectively induced by lactose and some low level by isoprophyl-$\beta$-D-thiogalactopyranoside(IPTG). When 30mM glucose was added at the different intervals to the culture that had been in contact with lactose, the different levels of the enzyme synthesis were observed. So, this suggests that glucose interfered with the entry of the lactose into the cells.The glucose inhibitory effect was not relieved by adding cAMP to the culture.

자연계에서 분리, 동정한 호알칼리성, 고온성 Bacillus sp. TA-11의 $\beta$-galactosidase 생합성 조절 기작을 조사 하였다. 시험균주의 $\beta$-galactosidase 생합성은 isoprophyl-$\beta$-Dthiogalactopyranoside (IPTG) 보다 lactose에 의해 더욱 효과적으로 유도 되었고 glucose는 lactose의 세포내 유입을 방해하면서 $\beta$-galactosidase의 생합성을 억제 하였다. 또한 이러한 glucose의 효소합성 억제효과는 cAMP에 의해 완하되지 못했다.

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