Clinical and Experimental Reproductive Medicine
- 제19권2호
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- Pages.153-162
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- 1992
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- 2233-8233(pISSN)
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- 2233-8241(eISSN)
냉동보존된 햄스터 난자를 이용한 인간정자의 생식력 평가
Assessment of Fertilizing Capacity of Human Spermatozoa Using Cryopreserved Hamster Oocytes
-
방명걸
(서울대학교 의과대학 산부인과학교실) ;
- 정구민 (서울대학교 의과대학 산부인과학교실) ;
-
김석현
(서울대학교 의과대학 산부인과학교실) ;
- 신창재 (서울대학교 의과대학 산부인과학교실) ;
-
김정구
(서울대학교 의과대학 산부인과학교실) ;
- 문신용 (서울대학교 의과대학 산부인과학교실) ;
-
이진용
(서울대학교 의과대학 산부인과학교실) ;
-
장윤석
(서울대학교 의과대학 산부인과학교실)
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Pang, Myung-Geol
(Department of Obstetrics and Gynecology, College of Medicine, Seoul National University) ;
- Chung, Ku-Min (Department of Obstetrics and Gynecology, College of Medicine, Seoul National University) ;
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Kim, Seok-Hyun
(Department of Obstetrics and Gynecology, College of Medicine, Seoul National University) ;
- Shin, Chang-Jae (Department of Obstetrics and Gynecology, College of Medicine, Seoul National University) ;
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Kim, Jung-Gu
(Department of Obstetrics and Gynecology, College of Medicine, Seoul National University) ;
- Moon, Shin-Yong (Department of Obstetrics and Gynecology, College of Medicine, Seoul National University) ;
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Lee, Jin-Yong
(Department of Obstetrics and Gynecology, College of Medicine, Seoul National University) ;
-
Chang, Yoon-Seok
(Department of Obstetrics and Gynecology, College of Medicine, Seoul National University)
- 발행 : 1992.12.31
초록
To solve the logistical problems of the sperm penetration assay (SPA) to provide just a sufficient number of hamster ova exactly when they are needed, a new method to cryopreserve the ova has been devised (1-step dehydration and 2-step thawing). After freezing & thawing of zona-intact (ZI) and zona-free (ZF) hamster ova according to this new method, the frozen-thawed ova were compared with fresh, control ova (FO) in terms of the degree of sperm penetration in SPA using semen samples from fertile donors, subfertile, and infertile male. Each sperm sample was capacitated for 42 hours inTEST-Yolk Buffer before insemination in SPA. In fertile doner, both the penetration rate and penetration index were lower in SPA using frozen ova (ZI; 92.4%, 6.2, ZF; 63.7%, 3.9) than those of SPA using fresh ova (99.3%, 8.4). There was a significant correlation between the penetration index of SPA using FO and ZI (p<0.001), and between those of SPA using FO and ZF and ova (p<0.001). In subfertile patient, both the penetration rate and penetration index were lowered in frozen ova (ZI; 62.3%, 1.3, ZF; 21.8%, 0.4) than those of fresh ova (74.8%, 1.8). There were significant correlation between the penetration rate and penetration index in ZI ova (p<0.05 and p<0.001, respectively). In infertile patient, both the penetration rate and penetration index were ZI; 3.1%, 0.0, ZF;0.0%, 0.0, respectively. There were significant correlation between the penetration rate and penetration index in ZI ova (p<0.05).
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