Clinical and Experimental Reproductive Medicine
- Volume 19 Issue 2
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- Pages.143-152
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- 1992
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- 2233-8233(pISSN)
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- 2233-8241(eISSN)
Cryopreservation of Zona-intact/-free Hamster Oocytes;Effect of 1-Step Equilibration and 2-Step Thawing
투명대 존재/부재 햄스터 난자의 동결보존;1-단계 평형과 2-단계 융해의 효과
- Chung, K.M. (Department of Obstetrics and Gynecology, College of Medicine, Seoul National University) ;
- Pang, M.G. (Department of Obstetrics and Gynecology, College of Medicine, Seoul National University) ;
- Kim, S.H. (Department of Obstetrics and Gynecology, College of Medicine, Seoul National University) ;
- Shin, C.J. (Department of Obstetrics and Gynecology, College of Medicine, Seoul National University) ;
- Kim, J.G. (Department of Obstetrics and Gynecology, College of Medicine, Seoul National University) ;
- Moon, S.Y. (Department of Obstetrics and Gynecology, College of Medicine, Seoul National University) ;
- Lee, J.Y. (Department of Obstetrics and Gynecology, College of Medicine, Seoul National University) ;
- Chang, Y.S. (Department of Obstetrics and Gynecology, College of Medicine, Seoul National University)
- 정구민 (서울대학교 의과대학 산부인과학교실) ;
- 방명걸 (서울대학교 의과대학 산부인과학교실) ;
- 김석현 (서울대학교 의과대학 산부인과학교실) ;
- 신창재 (서울대학교 의과대학 산부인과학교실) ;
- 김정구 (서울대학교 의과대학 산부인과학교실) ;
- 문신용 (서울대학교 의과대학 산부인과학교실) ;
- 이진용 (서울대학교 의과대학 산부인과학교실) ;
- 장윤석 (서울대학교 의과대학 산부인과학교실)
- Published : 1992.12.31
Abstract
The present experiments were focussed to modify a short slow-cooling protocol used for freezing of early stage embryo(Testart et al., 1986) and also to apply the modified method for the cryopreservation of hamster oocytes with Zona or without. The protocol was modified by changing the 4-step equilibration into 1-step and the 1-step thawing into 2-step. The oocytes were added in 1.5M PROH and 0.1M Sucrose, seeded at
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