Korean Journal of Environmental Agriculture (한국환경농학회지)
- Volume 11 Issue 1
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- Pages.67-76
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- 1992
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- 1225-3537(pISSN)
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- 2233-4173(eISSN)
Mechanism of Cadmium Accumulation in the Cell of Cadmium-Tolerant Bacterium, Pseudomonas putida
카드뮴내성균(耐性菌)(Pseudomonas putida)에 의한 균체내(菌體內) 카드뮴 축적(蓄積) 기작(機作)
- Heo, Jong-Soo (Department of Agricultural Chemistry, College of Agriculture, Gyeongsang National University) ;
- Cho, Ju-Sik (Department of Agricultural Chemistry, College of Agriculture, Gyeongsang National University) ;
- Han, Mun-Gyu (Department of Agricultural Chemistry, College of Agriculture, Gyeongsang National University)
- Published : 1992.06.30
Abstract
A mechanistic study by which Cadmium-tolerant P.Putida C1 accumulates high conc of Cd in its cell body was performed. Approximately 57% Cd accumulated was distributed on the cell wall and the other 43% portion was in cytoplasm. 84% Cd of the Cd in the cell wall fractions present in the polyphosphate-polysaccharide fractions, but most of Cd in the cytoplasm fraction was in protein and nucleic acid. Cadmium affected the protein synthesis in P. Putida. The intracellular protein content was decreased by cadmium addition, but the soluble protein precipitated by ammonium sulfate(
카드뮴내성균인 Pseudomonas Putida C1의 세포 내 카드뮴 축적기작을 구명하기 위하여 균체내 축적된 카드뮴의 세포 구성성분별 분포도, 단백질 함량변화 및 세포구성물질의 변화를 조사한 결과, 세포내에 축적된 카드뮴의 약 57%는 cell wall에 분포되어 있었으며 약 43%는 cytoplasm에 분포되어 있었다. Cytoplasm에 축적된 카드뮴은 protein과 nucleic acid 두 성분 모두에 분포되어 있었으며, cell wall에 축적된 카드뮴의 약 84% 이상이 polyphosphate-polysaccharide fraction에 분포되어 있는 것으로 나타났다. 세포내 protein 합성에 미치는 카드뮴의 영향을 조사한 결과, 카드뮴 무첨가배지에서 생장한 균체와 카드뮴이 함유된 배지에서 생장한 균체의 세포내 protein 함량은 카드뮴을 첨가함으로써 감소되었으나, ammonium sulfate(
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