Determination of the Structure of 5S rRNA from Xanthomonas citri

Xanthomonas citri의 5S rRNA 의 구조 결정

  • Bongrae Cho (Department of Chemistry, Seoul National University) ;
  • Myung-Un Choi (Department of Chemistry, Seoul National University) ;
  • Se Won Seh (Department of Chemistry, Seoul National University) ;
  • ahei Ihm (Department of Chemistry, Kyunggi University) ;
  • Moonjoo Koh (Department of Chemistry, Chosun University) ;
  • Inwon Park (Department of Chemistry, Seoul National University)
  • 조봉래 (서울대학교 자연과학대학 화학과) ;
  • 최명언 (서울대학교 자연과학대학 화학과) ;
  • 서세원 (서울대학교 자연과학대학 화학과) ;
  • 임자혜 (경기대학교 화학과) ;
  • 고문주 (조선대학교 화학과) ;
  • 박인원 (서울대학교 자연과학대학 화학과)
  • Published : 1992.06.20

Abstract

The structure of th 5S rRNA isolated from Xanthomonas citri was determined by enzymatic and chemical degradation methods. It consists of 119 nucleotides and contains no modified nucleosides. As does the 5S rRNA of X. maltophilia, it contains an additional uridine residue on the 5'-terminus. The secondary structure of the 5S rRNA from X. citri was almost identical to the generalized models proposed by many other workers [De Wachter et al., Biochimie, 64, 311 (1982); Specht et al., Nucleic Acids Res., 18, 2215 (1990); Cho et al., Proceedings of the First Symposium on Biomolecules, p. 9 (1991)]. The secondary structure of 5S rRNA from X. citri consists of five helices, five loops and two bulges. This 5S rRNA has a uridine residue at position 66 as a bulge.

Xanthomonas citri의 5S rRNA를 분리, 정제하여 효소적 방법과 화학적 방법으로 그 구조를 결정하였다. 이 5S rRNA는 119개의 누클레오티드로 구성되어 있으며 변형된 누클레오시드를 함유하지 않는다. 그리고 이 5S rRNA는 X. maltophilia의 것처럼 5'-말단에 가외의 우리딘 잔기를 하나 더 가지고 있다. 결정한 X. citri의 5S rRNA의 이차구조는 다른 원핵세포의 것들에 대해서 제안된 일반 모형들과 매우 유사하며 [De Wachter et al., Biochimie, 64, 311 (1982); Specht et al., Nucleic Acids Res., 18, 2215 (1990); Cho et al., Proceedings of the First Symposium on Biomolecules, p. 9 (1991)], 5개의 이중나선 줄기와 5개의 단일가닥 고리 그리고 2개의 내밀린 구조를 가진다.

Keywords

References

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