Bulletin of the Korean Chemical Society

Korean Chemical Society (대한화학회)
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An Approach to Isolation of Thromboxane Synthase (TX-SYN) by Ligand Tethered Affinity Techniques

  • Andersen Niels H. (Department of Chemistry, University of Washington) ;
  • Rhee Jaekeol (Dong-A Pharmaceutical Company Lab.)
  • Published : 1992.04.20
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Abstract

The affinity chromatographic technique was applied to the isolation of Thromboxane Synthase, with a variety of imidazolyl alkanoic acids coupled Sepharose 2B including a gel (G in Table 4) which has one free COOH group in the bound affinity ligand. The effect of ligand structure on the "affinity" and "selectivity" for thromboxane synthase isolation is described.

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References

  1. Ph. D Thesis, University of Washingnton Rhee. Jk
  2. Modern Phamacology Craig, Charles R.;Stitzel, Robert E.
  3. Nature(London) v.261 P. Neediman;S. Moncada
  4. Prostaglamdins v.12 S. Moncada;P. Needlman;S. Bunting;JR Vane
  5. J. Biol. Chem. v.252 T. Yoshimoto;S. Yamamoto;M. Okuma;O. Hayaishi
  6. J. Biol. Chem. v.260 M. Haurand;V. Ullrich
  7. Adv. Prostaglandins and Thromboxane Research v.11 V. Ullrich;M. Haurand
  8. Bull. Korean. Chem. Soc. v.13
  9. J. Med. Chem. v.30 P. W. Manley;N. M. Allanson;R. G. Booth;DP. Tuffin
  10. Arch. Biochem. Biophys. v.216 CA. Mullin;B. D. Mammock
  11. Int. J. Biol. Macromol. v.1 K. Pommerenig;M. Kuhn;W. Jung;M. Benes
  12. Methods in Enzymology v.86 T. Yoshimoto;S. Yamamoto
  13. Biochem. Biophys. Res. Commun. v.74 F. F. Sun
  14. Affinity chromatography, A series of Monographs v.33 P. Mohr;K. Pommerenig
  15. Anal Biochem v.72 MM. Bradford
  16. Adv. Prostaglandins and Thromboxane Research v.6 S. Hammarstrom;U. Diczfalusy