Selection of an Ethanol Tolerant Clostridium thermohydrosulfuricum Strain

  • PARK, YOUNG-MIN (Metabolic Engineering Lab., Genetic Engineering Research Institute) ;
  • CHUL-HO KIM (Metabolic Engineering Lab., Genetic Engineering Research Institute) ;
  • SANG-KI RHEE (Metabolic Engineering Lab., Genetic Engineering Research Institute)
  • Published : 1992.09.01

Abstract

An ethanol tolerant mutant was selected by successive transfers of Clostridium thermohydrosulfuricum ATCC 33223 into the media with progressively higher ethanol concentrations. The growth kinetics of the mutant were characterized under various growth conditions. Physiological differences such as enhanced growth, tolerance to various solvents, alteration of the optimum temperature and the ratio of end products during fermentation were noticed in the mutant.

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