Journal of Microbiology and Biotechnology
- Volume 2 Issue 1
- /
- Pages.21-25
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- 1992
- /
- 1017-7825(pISSN)
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- 1738-8872(eISSN)
Production and Purification of Soluble Recombinant Human Lymphotoxin in Escherichia coli
- Choi, Yoon-Ho (Department of Dairy Science, Sung Kyun Kwan University) ;
-
Na, Doe-Sun
(College of Medicine, University of Ulsan)
;
- Pan, Jae-Gu (Genetic Engineering Research Institute, Korea Institute of Science and Technology) ;
- Park, Seung-Kiel (Genetic Engineering Research Institute, Korea Institute of Science and Technology) ;
- Yoo, Hyang-Sook (Genetic Engineering Research Institute, Korea Institute of Science and Technology) ;
-
Kang, Kook-Hee
(Department of Dairy Science, Sung Kyun Kwan University)
;
- Sung, Moon-Hee (Genetic Engineering Research Institute, Korea Institute of Science and Technology)
- Published : 1992.03.01
Abstract
Human lymphotoxin (LT) was produced in E. coli as a soluble protein. The level of recombinant human LT production was about 4% of the total souble proteins of E. coli extracts. Recominant human LT was purified to apparant homogeneity by a simple procedure utilizing FPLC on Mono Q and Mono S columns. The specific activity of the purified LT was