Properties of Chitobiase Produced by Streptomyces sp.

Strepsomyces속 균주가 생산한 Ghitobiase의 효소학적 성질

Streptomyces sp. YB-88-20 was Isolated from soil and the properties of chitobiase were investigated. The optimal reaction condition for the enzyme was pH 5.5 and 4$0^{\circ}C$ , and was stable in the range of pH 4. 0 to 5.5 and temperature at 4$0^{\circ}C$, and 40 min, respectively The enzyme was inactivated by heating at 45$^{\circ}C$ for 1 hr. The enzyme was slightly activated by Mna+. Mg2+, but inhibited by Fea+. Km and activation energy was 1.5072 M and 8.314 kcal/mol.