The Korean Journal of Zoology (한국동물학회지)

The Korean Society for Integrative Biology (한국통합생물학회)
권호 표지

Purification and Characterization of Storage Protein-1 from Galleria mellonella

꿀벌부채명나방(Galleria mellonella L.)의 저장단백질-1(storage protein-1)의 정제 및 물리화학적 연구

  • 이용호 (고려대학교 이과대학 생물학과) ;
  • 여성문김학열 (고려대학교 이과대학 생물학과 단국대학교 자연과학대학 생물학과)
  • Published : 1992.10.01
Download PDF
⟨ Previous Next ⟩

Abstract

Storage protein-1 (SP-1) of Gallerio mellonella was identified in hemolvmph and fat body by electrophoresis. SP-1 was purified from hemolvmph by KBr density gradient ultracentrifugation , DEAE-cellulose (DE52) ion-exchange chromatography, and gel permeation chromatography (Sephadex G-200). Purity of SP-1 was confirmed by Non-SDS PAGE and electron microscope. SP-1 is 9.4 nm in diameter and regular octahedron in shape. SP-1 has isoelectric point of 5.7 and native molecular weight of 365 K dalton and is composed of one type of subunit with molecular weight of 82 K dalton. Ttiacylslvcerol and phospholipid were found to be maior lipid components in SP-1.

Keywords