The Korean Journal of Zoology (한국동물학회지)
- Volume 35 Issue 1
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- Pages.17-22
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- 1992
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- 0440-2510(pISSN)
Isolation and Partial Chemical Characterization of the Yolk Proteins from Drosophila sp. (robusta species group)
Drosophila sp.(robusta species group)의 난황 단백질의 분리 및 부분적 화학적 특성
- Kim, Se-Jae (Department of Biology, Cheju National University, Cheju 690-756) ;
- Gi (Department of Biology and SRC for Cell, Dikerentiation, Seoul National Uniuenity, Seoul, 151-742, Depanment of Molecular Biology and SRC for Cell, Differentiation, Seoul National Uniuenity, Seoul 151-742)
- Published : 1992.01.01
Abstract
The three yolk polypeptides have been isolated and partially characterized. Their molecular weights of YPI, YP2, and YP3 were 48, 000, 47, 000, and 46, 000, respectivelv, as judged by SDS-polyacrvlamide gel electrophoresis. They have different digestion products upon in situ peptide mapping by limited proteolysis. Two-dimensional gel electrophoresis showed that their isoelectric points were heterogeneous from 5.92 to 6.54. And thew showed three different antigen-antibody reactions when each polvpeptides is reacted with antisera made to a mixture of all of three. These data reported here indicate that the yolk proteins are consisted of distinctive polypeptides in Drosophlla sp. (robusta species group).