Archives of Pharmacal Research
- Volume 15 Issue 3
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- Pages.196-203
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- 1992
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- 0253-6269(pISSN)
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- 1976-3786(eISSN)
Membrane-Ordering Effects of Barbiturates on Pure Phospholipid Model Membranes
- Knag, Jung-Sook (College of Pharmacy, Kyung Sung University) ;
- Chung, Young-Za (College of Pharmacy, Kyung Sung University) ;
- Cho, Goon-Jae (College of Dentistry, Pusan National University) ;
- Byun, Won-Tan (College of Dentistry, Pusan National University) ;
- Yun, Il (College of Dentistry, Pusan National University)
- Published : 1992.09.01
Abstract
Intramolecular excimer formation of 1, 3-di(1-pyrenyl)propane (Py-3-Py) and fluorescence polarization of 1, 6-diphenyl-1, 3, 5-hexatriene (DPH) were used to investigate the effects of barbiturates on the fluidity of model membranes of phosphatidycholine (SPMVPC), phosphatidylserine (SPMVPS), and phosphatidylinositol (SPMVPI) fractions of synaptosomal plasma membrane vesicles (SPMV) isolated from bovine cerebral cortex. In a dose-dependent manner, barbiturates decreased the excimer to monomer fluorescence intensity ratio (I'/I) of Py-3-Py and increased the anisotropy(r), rotational relaxation time (P), limiting anisotropy