Applied Biological Chemistry
- 제35권5호
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- Pages.410-414
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- 1992
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- 2468-0834(pISSN)
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- 2468-0842(eISSN)
Streptomyces sp. S56 endoinulase의 고정화 및 성질
Immobilization and properties of Streptomyces sp. S56 endoinulase
- Kim, Su-Il (Department of Agricultural Chemistry and Research Center for New Bio-Materials in Agriculture, Seoul National University) ;
- Chang, Ho-Jin (Department of Agricultural Chemistry and Research Center for New Bio-Materials in Agriculture, Seoul National University)
- 발행 : 1992.10.30
초록
Streptomyces sp. S56 endoinulase를 DEAE-cellulose에 고정화하였고 고정화 효소의 일반 특성을 유리효소와 비교하였으며 돼지감자 추출물 및 inulin의 고정화 효소에 의한 가수분해 조건을 조사하였다. 고정화 효소의 최적 작용 pH는
Endoinulase from Streptomyces sp. S56 was immobilized by adsorption on DEAE-cellulose in 0.01 M citrate-sodium phosphate buffer, pH 6.0 and the properties of immobilized and free enzymes were investigated. The immobilized enzyme preparation, having 40 inulase activity units per dried matrix, revealed the maximal activity at