Applied Biological Chemistry
- Volume 35 Issue 4
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- Pages.308-314
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- 1992
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- 2468-0834(pISSN)
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- 2468-0842(eISSN)
Extraction, purification and properties of anti-complementary polysaccharide from Arecae Pericarpium
대복피로부터 항보체 활성다당의 추출, 정제 및 그 특성
- Kwon, Kyung-Sup (Department of Food Technology, Korea University) ;
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Shin, Kwang-Soon
(Department of Food Technology, Korea University) ;
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Cho, Hong-Yon
(Department of Food Science and Industry, Korea University) ;
- Yang, Han-Chul (Department of Food Technology, Korea University)
- Published : 1992.08.30
Abstract
To examine the characteristics of anti-complementary compounds from Arecae Pericarpium (the pericarps of Areca catechu) which showed the highest activity during our screening procedures, the extraction and purification were performed. AC-1 fraction from Arecae Pericarpium was purified by hot water extraction, methanol reflux, ethanol precipitation, dialysis and lyophilization. This compound had total sugar 48.2%, uronic acid 14.6% and protein 36.8%. Rhamnose, arabinose, mannose and galactose were found in sugar components. By cetavlon (cetyltrimethylammonium bromide) treatment AC-1 was fractionated to AC-2, AC-3 and AC-4. Among them, AC-2 showed the highest activity and yield. By periodate oxidation, AC-2 was deactivated, but had no change in activity by pronase digestion. Moreover active fractions, AC-2-IIIa and AC-2-IIIc isolated from AC-2 by two successive column chromatography using DEAE-Toyopearl
선별과정 중 가장 높은 항보체 활성을 보였던 대복피를 대상으로 활성물질의 특성을 조사하기 위하여 추출 및 정제를 행하였다. 대복피를 열수추출, methanol 환류 및 ethanol 침전을 행한 후, 투석 및 동결건조를 거쳐 활성획분 AC-1을 얻었는 바, 이들은 총당 48.2%, 산성당 14.6%, 단백질 36.8%로 구성되어 있었고 주구성당으로 rhamnose, arabinose, mannose 및 galactose를 함유하고 있었다. 이들은 다시 cetavlon(cetyltrimethylammonium bromide) 처리에 의해 AC-2, AC-3, AC-4로 분획되었으며 특히 AC-2의 경우 활성과 수율면에서 우수 하였다. 이들은 periodate 산화에 의해 활성의 감소를 일으킨 반면 pronase 소화에 의해서는 활성의 변화가 없는 특성을 보여 주었다. 더우기 AC-2는 DEAE-Toyopearl 650C(