Applied Biological Chemistry
- 제35권4호
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- Pages.237-241
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- 1992
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- 2468-0834(pISSN)
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- 2468-0842(eISSN)
Halomonas sp. ES 10이 생산하는 alkaline protease의 특성
Characteristics of the alkaline protease from the moderate halophile, Halomonas sp. ES 10
- Kim, Chan-Jo (Department of Food Technology, Chungnam National University) ;
- Oh, Man-Jin (Department of Food Technology, Chungnam National University) ;
- Choi, Seong-Hyun (Department of Food Technology, Chungnam National University)
- 발행 : 1992.08.30
초록
Halomonas sp. ES 10이 생산하는 protease를 methanol 침전, Sephadex G-150, G-200 및 DEAE-Sephadex A-50으로 여과하여 비활성이 1,014 units/mg protein, 수율이 7%로 정제하였다. 이 효소의 작용 최적온도 및 pH는
The protease from Halomonas sp. ES 10 was purified by methanol precipitation, gel filtration on Sephadex G-150 and G-200, and ion exchange chromatography on DEAE-Sephadex A-50. The purified enzyme was found to be homogeneous by polyacrylamide gel electrophoresis. The specific activity of purified enzyme was 1,014 units/mg protein, and the yield of the total activity from the culture filtrate was 7%. The optimal temperature and pH for the enzyme activity were