Analysis of the Coumarin Constituents in Peucedanii Radix

식방풍중의 Coumarin성분의 확인 및 정량

  • Shin, Kuk-Hyun (Natural Products Research Institute, Seoul National University) ;
  • Kang, Sam-Sik (Natural Products Research Institute, Seoul National University) ;
  • Chi, Hyung-Joon (Natural Products Research Institute, Seoul National University)
  • 신국현 (서울대학교 천연물과학연구소) ;
  • 강삼식 (서울대학교 천연물과학연구소) ;
  • 지형준 (서울대학교 천연물과학연구소)
  • Published : 1992.03.30

Abstract

A new method for the analysis of coumarin constituents in the roots of Peucedanum japonicum by high performance liquid chromatography was established. Among two coumarin constituents identified, peucedanol was confirmed to be applicable to a standard compound. A reversed phase system with a ${\mu}Bondapak$ C_{18}$, column using $H_2O-MeOH=5\;:\;4$ as a moble phase was developed. Peucedanol and a minor constituent, umbelliferone were detected at 333nm and the analysis was successfully carried out within 20 min.

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