The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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Formation of Filamentous Crystal in Transformants of Pleurotus species

느타리버섯 형질전환주(形質轉換株)에서 Filamentous Crystal 형성(形成)

  • Byun, Myung-Ok (Applied Mycology and Mushroom Division. Agricultural Sciences Institute R. D. A.) ;
  • Cha, Dong-Yeul (Applied Mycology and Mushroom Division. Agricultural Sciences Institute R. D. A.)
  • 변명옥 (농촌진흥청 농업기술연구소 균이과) ;
  • 차동열 (농촌진흥청 농업기술연구소 균이과)
  • Published : 1992.09.30
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Abstract

Aerial crystalline filaments emerged from dense type of mycelia on some monokaryotic transformants of Pleurotus florida, P. ostreatus and P. sajorcaju. Cytstals were not dissolved in water but soluble in ethanol or at the temperature of higher than $80^{\circ}C$. Crystals were detected in the mycelia grown on the mushroom minimal medium as well as the mushroom complete medium. They were produced on both liquid media and agar plate. Although the mycelia incubated at $15-20^{\circ}C$, produced crystals, the mycelia incubated at $30-35^{\circ}C$ did not. Furthermore, crystal forming mycelia were obtained from monokaryotic basidiospore of P. ostreatus and P. sajor-caju by mutations (UV irradiation).

사철느타리버섯, 느타리버섯, 여름느타리버섯 형질전환주(形質轉煥珠)에서 crystal이 형성(形成) 되었으며, crystal은 물에는 녹지않고 고온(高溫)이나 ethanol에 녹으며 ethanol이 휘발되면 재결정이 이루어졌다 . crystal은 고체(固體)배지뿐 아니라 액체(液體) 배지(培地)에서도 형성(形成)되며 균사(菌絲)를 $15-25^{\circ}C$에서 배양(培養)할때 형성(形成)되나 $30-35^{\circ}C$ 에서는 형성(形成)되지 않았다. 또한 uv 를 이용(利用)하여 돌연변이(突然變異)를 유발(誘發)시켰을때도 여름느타리 버섯과 느타리버섯의 1핵(核) 균사(菌絲)에서 crystal을 형성(形成)하는 균주(菌株)를 얻었다 .

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