한국균학회지 (The Korean Journal of Mycology)
- 제20권1호
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- Pages.72-76
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- 1992
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- 0253-651X(pISSN)
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- 2383-5249(eISSN)
구름버섯 배양액으로부터 단백다당류의 추출 및 정제방법
Extraction and Separation of Protein-bound Polysaccharide Produced by Coriolus versicolor (Fr) Quel
- Park, Kyung-Sook (Department of Food Science and Technology, Yeungnam University) ;
- Lee, Jae-Yang (Department of Biochemistry, Yeungnam University) ;
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Lee, Sang-Jik
(Department of Biochemistry, Yeungnam University) ;
- Kim, Seon-Hee (Department of Food Science and Technology, Yeungnam University) ;
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Lee, Jae-Sung
(Department of Food Science and Technology, Yeungnam University)
- 발행 : 1992.03.31
초록
고등균류의 배양액 및 균사체로부터 항암작용이 있는 단백다당류의 추출 및 정제방법에 관한 실험을 구름버섯 균사배양체를 이용하고 실시하였다. 실온에서 비이온성 계면활성제인 2% Triton X-100과 함께 교반시키면서 추출했을 때 얻어진 단백다당류의 함량이 이미 사용되어 온
The extraction and separation methods of protein-bound polysaccharides from the mycelium and culture broth of Coriolus versicolor (Fr) Quel were investigated. The use of 2% solution of surface active agent Triton X-100, was effective for extraction of the protein-bound polysaccharides from the mycelium. For the separation and partial purification of the protein-bound polysaccharides, the column chromatography using DEAE-Cellulose and DEAE-Sephadex proved to be effective.