대한약리학회지 (The Korean Journal of Pharmacology)
- 제28권2호
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- Pages.163-170
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- 1992
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- 0377-9459(pISSN)
허혈재-관류 적출심장에서 Arachidonic Acid에 의한 산소래디칼 생성 및 심근손상에 대한 Cyclobuxine의 영향
Effect of Cyclobuxine on Oxygen Free Radical Production and Cellular Damage Promoted by Arachidonate in Perfused Rat Hearts
- 이종화 (순천향대학교 의과대학 약리학교실) ;
- 권준택 (순천향대학교 의과대학 약리학교실) ;
- 조병헌 (순천향대학교 의과대학 약리학교실) ;
- 박종안 (순천향대학교 의과대학 약리학교실) ;
- 김유재 (순천향대학교 의과대학 약리학교실) ;
- 김종배 (순천향대학교 의과대학 약리학교실) ;
- 차영덕 (순천향대학교 의과대학 약리학교실) ;
- 김창호 (순천향대학교 의과대학 약리학교실)
- Lee, Jong-Hwoa (Department of Pharmacology, College of Medicine, Soonchunhyang University) ;
- Kwon, Jun-Tack (Department of Pharmacology, College of Medicine, Soonchunhyang University) ;
- Cho, Byung-Heon (Department of Pharmacology, College of Medicine, Soonchunhyang University) ;
- Park, Jong-An (Department of Pharmacology, College of Medicine, Soonchunhyang University) ;
- Kim, Yu-Jae (Department of Pharmacology, College of Medicine, Soonchunhyang University) ;
- Kim, Jong-Bae (Department of Pharmacology, College of Medicine, Soonchunhyang University) ;
- Cha, Young-Deog (Department of Pharmacology, College of Medicine, Soonchunhyang University) ;
- Kim, Chang-Ho (Department of Pharmacology, College of Medicine, Soonchunhyang University)
- 발행 : 1992.12.30
초록
흰쥐의 허혈-재관류 적출심장에서 arachidonic acid의 투여에 의해 촉진된 superoxide anion의 생성과 심근손상에 대한 cyclobuxine (스테로이드성 알카로이드)의 영향을 관찰하였다. 적출심장을 Langendorff 관류장치에 현수하고 0.5 ml/min의 저용량으로 45분간 관류한 후 정상관류 (7ml/min)로 복귀시켜 허혈-재관류 심장으로 사용하였다. 재관류 시 arachidonate (0.1과
The present study was attempted to investigate the effect of cyclobuxine (a steroidal alkaloid) on generation of reactive oxygen metablite and myocardial damage promoted by an exogenous administeration of arachidonate in ischemic-reperfused hearts. Langendorff preparation of the isolated rat heart was made ischemic condition by reducing the flow rate to 0.5 ml/min for 45 min, and then followed by normal reperfusion (7 ml/min) for 5 min. The generation of superoxide anion was estimated by measuring the SOD-inhibitable ferricytochrome C reduction. The degree of lipid peroxidation in myocardial tissue was estimated from the tissue malondialdehyde (MDA) concentration using thiobarbituric acid method. The myocardial cell damage was observed by measuring LDH released into the coronary effluent. Sodium arachidonate