YAKHAK HOEJI (약학회지)
- Volume 36 Issue 3
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- Pages.241-249
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- 1992
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- 0377-9556(pISSN)
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- 2383-9457(eISSN)
Purification and Characterization of A New Lectin from Marine Animal Lunella coronata coreensis
해양동물 눈알고둥으로부터 새로운 렉틴 성분의 분리 및 정제
- So, Myung-Suk (Institute for Drug Research, Yeungnam University) ;
- Suh, Young-Ah (Institute for Drug Research, Yeungnam University) ;
- Jeune, Kyung-Hee (College of Science, Yeungnam University) ;
- Chung, See-Ryun (Institute for Drug Research, Yeungnam University)
- Published : 1992.06.29
Abstract
The whole body extract of Lunella coronata coreensis agglutinated nonspecifically human and other animal erythrocytes. A new lectin was purified by the following procedures: 0.15 M NaCl extraction, salt fractionation, gel filtration, anionic and cationic ion exchange column chromatographies. Through these purification procedures, specific activity of LCC-I was increased from 276 to 9714.3 units/mg, And on polyacrylamide gel electrophoresis, LCC-I exhibited one major band. A molecular weight of LCC-I was assumed to be 20,000 by sodium dodesyl sulfate polyacrylamide gel electrophoresis. The purified lectin was relatively stable at various pH and heat. Among the tested sugars, lactose and lactulose inhibited lectin activity at a concentration of 6.25 mM, respectively.