Novel Phenol Sulfotransferase of Klebsiella K-36, Rat Intestinal Bacteria

흰쥐의 장내미생물로부터 분리한 새로운 페놀 설포트란스페라제

Klebsiella K-36 producing novel sulfotransferase was isolated from rat intestinal flora. The novel sulfotransferase catalyzed the transfer of sulfate group from p-nitrophenylsulfate to phenolic compounds but it did not use PAPS(3'-phosphoadenosine 5'-phosphosulfate) as a donor substrate. The present enzyme was 160 K daltons. Optimal pH was 10. When p-nitrophenyl sulfate was used as a donor substrate, 1-naphthol was the best substrate, followed by phenol, phenanthrol and tyrosine. The apparent Km for phenol using p-nitrophenylsulfate as a donor substrate and that for p-nitrophenylsulfate using phenol as an acceptor substrate were determined to be 0.66 mM and 0.11 mM, respectively.