Purification and Characterization of Complement System Activating Polysaccharide from the Bark of Kalopanax pictus N.

음나무 수피로부터 보체계 활성화 다당의 정제 및 특성

  • Shin, Keum (College of Natural Resources, Korea University) ;
  • Ra, Kyung-Soo (Institute of Biotechnology, Korea University) ;
  • Paik, Ki-Hyon (College of Natural Resources, Korea University)
  • 신금 (고려대학교 자연과학대학) ;
  • 라경수 (고려대학교 생물공학연구소) ;
  • 백기현 (고려대학교 자연과학대학)
  • Received : 1992.12.20
  • Published : 1992.12.30

Abstract

It was observed that the hot-water extract of the bark of Kalopanax pictus N. had the highest anti-complementary activity among the 11 kinds of forest materials. Methanol-and ethanol-soluble portions had low anti-complementary activities, but crude polysaccharide. HKP-0 had a high activity of 80%. HKP-0 contained 54.8% of total sugar and 27.9% of protein. The neutral sugars of HKP-0 consisted of mainly arabinose, galactose and glucose. HKP-4 fraction obtained by cetavlon treatment of HKP-0 showed the highest anti-complementary activity of 90%. The activity was not changed by pronase digestion bu decreased greatly by periodate oxidation. HKP-4 consisted of mainly arabinose and glucose with molar ratio of 1.0 : 22.4, HKP-4-I, an unabsorbed fraction from HKP-4 on DEAE Sepharose CL-6B column showed higher yield and activity than those of absorbed fractions. HKP-4-I was homogeneous, and its molecular weight was about 25,000. HKP-4-I contained 84.0% of neutral sugar and consisted of arabinose and glucose with molar ratio of 1.0 : 11.2. The anti-complementary activity of HKP-4-I was not decreased by the treatment of polymyxin B, and the polysaccharide activated both classical and alternative pathway in complement system. Void volume fraction obtained from HKP-4-I hydrolyzed with ${\alpha}$-amylase on Sephadex G-25 column only had a high anti-complementary activity.

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