한국잡초학회지 (Korean Journal of Weed Science)
- 제11권3호
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- Pages.195-204
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- 1991
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- 0253-7648(pISSN)
Glufosinate-ammonium에 대(對)한 수도(水稻)(Oryza sativa L.) 품종간(品種間) 반응(反應)
Responses of Rice Cultivars to Glufosinate-ammonium
- Hong, S.Y. (Department of Agronomy, Kyungpook National University) ;
- Kim, K.U. (Department of Agronomy, Kyungpook National University) ;
- Shin, D.H. (Department of Agronomy, Kyungpook National University)
- 발행 : 1991.09.30
초록
Glufosinate-ammonium에 대(對)한 수도(水稻) 품종간(品種間)의 반응차이(反應差異)를 구명(究明)하고자 glufosinate-ammonium에 대한 감수성(感受性) 반응(反應), 총(總) 수용성(水溶性) 단백질(蛋白質) 함량(含量), 단백질(蛋白質) 패턴의 변화(變化), glutamine synthetase의 활성(活性), 축적(蓄積)된 암모니아 함량(含量), 유리(遊離) 아마노산 조성변화(組成變化)를 조사(調査)를 실시(實施)한 바 결과(結果)를 요약(要約)하면 다음과 같다. 1. Glufosinate-ammonium 10ppm 에 대한 수도(水稻) 품종간(品種間) 반응(反應)을 보면 Fukei 126과 탐진벼의 신초(新鞘)의 길이가 무처리구(無處理區)에 비(比)해 각각(各各) 96%, 100%로 내성품종(耐性品種)으로, 영덕벼, 팔공벼, 남영벼는 신초(新鞘)의 길이가 무처리(無處理)에 비(比)해 각각(各各) 40%, 39%, 37%로 감수성품종(感受性品種)으로 분류(分類)하였다. 2. 총(總) 단백질(蛋白質) 함량(含量)은 내성품종(耐性品種)으로 선발(選拔)된 Fukei 126과 탐진벼는 무처리(無處理)에서 240.0, 207.2
This study was conducted to determine physiological responses rice cultivars to glufosinate-ammonium. Changes of total protein content, protein population, glutamine synthetase activity, accumulated ammonia content and free amino acid composition were examined in both tolerant and susceptible rice cultivars. Tamjinbyeo and Fukei 126 showed relatively tolerant response to glufosinate-ammonium while Namyeongbyeo, Palgongbyeo and Youngdugbyeo were susceptible to it. Total protein content of two tolerant cultivars was 93.2 of the untreated control in average but three susceptible cultivars showed 76.5 of the untreated control in average, showing 16.7 difference. Protein profiles of the tolerant cultivars seemed to be not affected by glufosinate-ammonium treatment. However, in susceptible cultivar like Namyeongbyeo, 10ppm of glufosinate-ammonium application resulted in decrease of band density or disapperance of spot density in near 20kD and in between 45kD and 66kD on 2D-PAGE. Glutamine synthetase activity in susceptible cultivars was markedly inhibited by glufosinate-ammonium treatment, accompanying remarkable increase of ammonia content by three times greater than tolerant cultivars, and markedly increase of glutamic acid, showing 430% of the untreated control.