대한수의학회지 (Korean Journal of Veterinary Research)
- 제31권3호
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- Pages.319-327
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- 1991
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- 2466-1384(pISSN)
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- 2466-1392(eISSN)
은행엽(銀杏葉)의 가토(家兎) 피부(皮膚) 접촉성염증(接觸性炎症) 유발(誘發) 독성물질(毒成物質)에 관(關)한 연구(硏究)
Studies on toxic substances of Gingko leaves inducing contact dermatitis in rabbit
- Baek, Byeong-kirl (College of Veterinary Medicine, Chunbuk National University) ;
- Kim, Byeong-su (College of Veterinary Medicine, Chunbuk National University) ;
- Kim, Sun-hee (College of Pharmacy, Chungnam National University) ;
- Ahn, Byung-zun (College of Pharmacy, Chungnam National University)
- 투고 : 1991.03.21
- 발행 : 1991.07.31
초록
Gingko biloba is one of the oldest trees on earth. It can be traced 300 million years or more into past. In recent years, this tree has become increasingly popular because of its longevity. Amount of comsumption of Gingko leaves in Korea is increased to produce drugs for blood circulating system's disorder. The workers who work at the company of product drug with Gingko leaves complain pruritus at belt position of west, however toxic substance from extracts of Gingko biloba was carried out to determine what kind of the toxic substance from extract could induce a contact dermatitis by means of histopathological study. Dried leaves of Korean G. biloba were exhaustively extracted with chloroform. The aqueous chloroform residue was extracted with hexane. After evaporation, the hexane extract was passed through a silica gel column chromatography, it was separated into 9 fractions by means of gradient solvent (benzene: acetone/ 98~93 : 2~7%). The 4th and 7th fraction were idendified as bilibol and gingkolic acid by silica gel thin layer chromatography and NMR spectrophotometer. The 4th and 7th fractions of Korean Gingko leaves have an irritant substances which can be induced ear swelling and increased thickness of stratum cornium of rabbit ear exposed with the fractions (3 time/3 day,