Effects of levamisole, selenium and tocopherol on the functions of blood neutrophil and peritoneal macrophage of Korean native goats

Levamisole, selenium 및 tocopherol이 한국재래산양의 혈중 호중구 및 복강 대식구의 기능에 미치는 영향

In this study, the immunomodulating effects of levamisole, selenium and tocopherol on blood neutrophils and peritoneal macrophages of goat were evaluated in vitro and in vivo. The functions of blood neutrophils and peritoneal macrophages were assayed by random and direct migration, phagocytosis of S aureus, production of superoxide and hydrogen peroxide. The results obtained were summarized as follows: In vitro trials 1. Levamisole treatment enhanced the random and direct migration of goat blood neutrophils when compared with untreated cell, and a significant (p<0.01) enhancement was noticed at the concentration of $100{\mu}g$ for direct migration and $50{\mu}g{\sim}1,000{\mu}g\;per\;ml$ of culture medium for random migration. There was no influence of selenium and tocopherol on random and direct migration of neutrophil at all of treatment concentration. 2. Neutrophils produced higher levels of superoxide by lcvamisole treatment at the concentration of $100{\mu}g$ and by selenium treatment at the concentration of $1.0{\mu}g$, but the production of hydrogen peroxide was not increased. Tocopherol had no effect on the production of antimicrobicidal oxygen metabolites of neutrophils at various concentrations. 3. No differences of phagocytic activity were observed when neutrophils were treated with three substances. In vivo trials 1. Blood neutrophils of goats orally administered levaraisole showed significantly (p<0.05) higher random migration from 2 to 24 hours after feeding (2.5mg/kg of body weight). Augmentation of random migration of neutrophil from goats orally administered selenium-tocopherol mixture (selenium $100{\mu}g$-tocopherol 200IU/head/day) was observed at 10 days and the significant (p<0.05) increase was shown from 30 days after feeding and continued throughout the feeding periods. 2. There was no effect on phagocytic activity and production of antimicrobicidal oxygen metabolites of neutrophils from goats administered levamisole or selenium-tocopherol mixture. 3. Random migration, production of superoxide and hydrogen peroxide and S aureus phagocytic activity of peritoneal macrophages of goats administered 300ml of levamisole-thioglycollatc medium mixture $(2.5{\mu}g/ml)$ into peritoneal cavity increased significantly (p<0.01 or p<0.05) when compared with those of goats administered thioglycollate medium alone.