대한수의학회지 (Korean Journal of Veterinary Research)
- 제31권1호
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- Pages.49-53
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- 1991
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- 2466-1384(pISSN)
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- 2466-1392(eISSN)
Phytomitogen에 의한 토끼 임파구의 blast transformation I. 유사분열에 미치는 배지, 유사분열촉진물질 및 배양시간의 효과
Phytomitogen induced blast transformation of rabbit I. Effect of medium, phytomitogen and culture hours on the uptake of 3H-thymidine
- Kim, Jong-shu (College of Veterinary Medicine, Gyeongsang National University) ;
- Kim, Chung-hui (College of Veterinary Medicine, Gyeongsang National University)
- 투고 : 1990.10.05
- 발행 : 1991.01.31
초록
The present study has been carried out to investigate the optimal condition on lymphocyte blastogenesis of rabbit lymphocytes, whole blood culture and microculture system in conjunction with a semiautomatic multiple sample harvester(SAMSH) was used to study the In vitro optimal condition of rabbit lymphocytes. Data were presented to show many variables that are involved in studying the phytohemagglutinin(PHA) and lipopolysaccharide(LPS) response of rabbit lymphocyte in a microculture system. Analysis indicated that the conditions for optimal PHA as measured by incorporation of