Korean Journal of Animal Reproduction (한국가축번식학회지)
- Volume 15 Issue 1
- /
- Pages.33-39
- /
- 1991
- /
- 1226-5284(pISSN)
Morphological Changes of Bovine Epididymal Spermatozoa Incubated In Chemical Defined Medium
人工培養液에서 培養된 牛精子의 形態的 變化
-
Go, Dae-Hwan
(Department of Animal Husbandry, Sang-Ji Junior College)
;
- Yun, San-Hyeon (Department of Animal Science, Kon-Kuk University) ;
- Eom, Gi-Bung (Department of Animal Science, Kon-Kuk University) ;
- Lee, Gyeong-Gwang (Genetic Engineering Center, KIST) ;
- Jeong, Gil-Saeng (Department of Animal Science, Kon-Kuk University)
-
고대환
(尙志大學校倂設專門大學 畜産科)
;
- 윤산현 (建國大學校 畜産學科) ;
- 엄기붕 (建國大學校 畜産學科) ;
- 이경광 (韓國科學技術硏究院 遺傳工學센타) ;
- 정길생 (建國大學校 畜産學科)
- Published : 1991.03.01
Abstract
This experiment was carried out to investigate ultrastructural changes that occurred in bovine epididymal spermatozoa during incubation in a BO medium supplemented with 5 mM caffeine. No structural changes were observed under electron microscope in the majority of non-incubated sperm(79.8%) keeping the membrane intact. Structural changes were however observed when spermatozoa were cultured in a BO medium supplemented with 5 mM caffeine, which were classified into 4 types, intact(29.4%), vesiculated(45.6%), acrosome lost(17.8%) and degenerated(7.2%) spermatozoa, respectively. These results indicated that : 1) vesiculation of spermatozoa membrane is normal acrosome reaction and acrosome lost of spermatozoa is dead one ; 2) caffeine can induce acrosome reaction of bovine epididymal spermatozoa.
본 試驗은 caffeine을 함유한 人工 培養液에서 培養한 牛精巢上體 精子의 形態的 變化를 透過 電子顯微鏡으로 관찰하였다. 電子顯微鏡에서 관찰한 결과 채취 직후의 無培養 精子는 대부분(79.8%)에서 구조상의 變化없이 원형 그대로의 原形質膜을 유지하고 있었으나 5 mM의 caffeine을 함유한 BO培養液에 3시간 培養한 精子는 尖體의 구조상 4가지 形態로 분류되었다. 즉 無反應, 胞狀化, 尖體離脫 및 退行으로서 각각 29.4%, 45.6%, 17.8% 및 7.2%였다. 이러한 결과는 精子膜의 胞狀化가 정상적인 尖體反應이며 尖體離脫 精子는 死滅精子라는 것과 caffeine이 牛精巢上體 精子의 尖體反應을 誘起시킬 수 있다는 것을 示唆하고 있다.
Keywords