Journal of the Korean Chemical Society (대한화학회지)

Korean Chemical Society (대한화학회)
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Cytidine Biosensor Using Bacteria and Organelle

Bacteria 및 Organelle을 이용한 Cytidine Biosensor

  • Ihn, Gwon Shik (Department of Chemistry, College of Natural Science, Keimyung University) ;
  • Kim Jeong-Suk (Department of Chemistry, College of Natural Science, Keimyung University) ;
  • Jeon Young Guk (Department of Chemistry, College of Natural Science, Keimyung University) ;
  • Kim Bong Weon (Department of Chemistry, College of Natural Science, Keimyung University)
  • 인권식 (계명대학교 자연과학대학 화학과) ;
  • 김정숙 (계명대학교 자연과학대학 화학과) ;
  • 전영국 (계명대학교 자연과학대학 화학과) ;
  • 김봉원 (계명대학교 자연과학대학 화학과)
  • Published : 1991.02.20
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Abstract

The cytidine bio-sensors have been constructed by immobilizing the bacterium Proteus mirabilis and organelle on an ammonia gas sensor. The bacterial sensor was investigated for the effects of pH, temperature, buffer solution, bacterial amounts, interferences and lifetime. The bacterial sensor had linearity in the range of 5.0 ${\times}$ 10$^{-4}$M ∼ 1.0 ${times}$ 10$^{-2}$M cytidine with a slope of 56 mV/decade at pH 7.8, 30$^{\circ}C$ and 3 mg in 1.0 M phosphate buffer solution. This bacterial sensor was compared with it's organelle sensor.

Proteus mirabilis균과 organelle를 NH$_3$ 기체감응전극에 고정시켜 cytidine에 대한 바이오센서를 제조하였다. 박테리아 센서에 대한 pH, 온도, 완충용액, 균량 및 방해물질의 영향과 수명을 조사하였다. 그 결과 0.1M phosphate 완충용액에서 pH 7.8, 온도 30$^{\circ}C$, 균량 3mg이었을 때, 5.0 ${times}$ 10$^{-4}$M ∼ 1.0 ${times}$ 10$^{-2}$M cytidine 직선범위 내에서 56 mV/decade 의 감응기울기를 나타내었다. 이 박테리아 전극의 감응특성을 organelle 전극의 경우와 비교하였다.

Keywords

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