Bulletin of the Korean Chemical Society

Korean Chemical Society (대한화학회)
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Anomalous Absorbance-Temperature Profile of Calf Thymus DNA in Presence of Spermine

  • Chan-Yong Lee (Department of Biochemistry, Chungnam National University) ;
  • Hyeong-Won Ryu (Department of Biochemistry, Chungnam National University) ;
  • Moon-Jip Kim (Department of Physics, Soonchunhyang University) ;
  • Thong-Sung Ko (Department of Biochemistry, Chungnam National University)
  • Published : 1991.06.20
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Abstract

An anomalous absorbance-temperature profile of calf thymus DNA, having a hypochromic trough just before the rise the $T_m$-region phase, occurs at the spermine concentration where the DNA collapses into a compact structure. The trough phase can be eliminated by the addition of ethidium bromide and also by a hydrophobic environment.

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References

  1. Nucleic Acids Res. v.11 K. A. Mark;G. C. Ruben
  2. Biochemistry v.18 R. W. Wilson;V. A. Bloomfield
  3. J. Mol. Biol. v.144 J. Widom;R. L. Baldwin
  4. Biopolymers v.20 S. A. Allison;J. C. Herr;J. M. Schurr
  5. Advances in Polyamine Research v.1 R. A. Campbell(et al.)(ed.)
  6. J. Mol. Biol. v.121 O. K. Chattoraj;L. C. Gosule;J. A. Schellman
  7. Nature v.259 L. C. Gosule;J. A. Schellman
  8. Proc. Natl. Acad. Sci. USA v.74 M. W. Hsiang;R. D. Cole
  9. Introduction to the Polyamines S. S. Chon
  10. Q. Rev. Biophys. v.11 G. S. Manning
  11. Biochem. J v.103 L. Stevens
  12. Biochemistry v.3 H. Tabor
  13. Physical Chemistry of Nucleic Aicds V. A. bloomfield;D. M. Crothers;I. Tinoco, Jr.
  14. J. Korean Chem. Soc. v.28 T. -S. Ko;J. Huh
  15. Nucleic Acids Res. v.1 M. Noll
  16. Nature v.255 F. H. C. Crick;A. Klug
  17. Biochim. Biophys. Acta v.741 K. A. Marx;T. C. Reynolds
  18. Angew. Chemie. v.82 J. Engel;G. Schwarz