Biochemical Characteristics of the Granulosis Viruses DNA of Common Cabbage Worm, Pieris rapae and Pieris brassicae

배추흰나비 과립병바이러스 DNA의 생화학적 특성

This study was carried out to acquire some basic biochemical informations on the granulosis virus (GV) DNA of Pieris rapae and Pieris brassicae. The thermal denaturation temperature (Tm) and G+C content of the DNA of the viruses were $83.7^{\circ}C$ and 35.5% for P. rapae GV, $84.0^{\circ}C$ and 35.9% for P. brassicae GV, respectively. There were some differences in the DNA fragmentation patterns of the two GV's produced by digestion with restriction endonucleases such as EcoR I , BamH I and Hind m . The homololgy between the two DNAs was caculated to be 97.0%. The size of the genome was estimated to be 103 kbp for P. rapae GV and 108 kbp for P. brassicae GV.

배추흰나비 P. rapae와 P. brassicae GV의 DNA성상과 이를 기초로한 상동성을 검정한 결과 Tm값에서는 P. rapae GV DNA는 $83.7^{\circ}C$이고, P. brassicae GV DNA의 경우는 $84.0^{\circ}C$로 G+C 함량은 전자가 35.5%, 후자가 35.9%였다. 그리고 제한산소처리에 의한 DNA의 분석에서는 P. Rapae GV의 genome의 크기는 103 kb, P. brassicae GV DNA는 108kb로 나타났으며, 두바이러스 간의 상동성은 97.0%였다.