한국연초학회지 (Journal of the Korean Society of Tobacco Science)
- 제13권2호
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- Pages.34-41
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- 1991
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- 1225-2131(pISSN)
옥수수 전이인자 Ac가 도입된 연초조직의 재분화
Regeneration of Tobacco Tissue Introduced with the Maize Transposable Element Activator
초록
To explore the possibility of introducing Zea mays transposable element Ac(activator) which can be used as a mutagen and gene tag in tobacco plants other than maitre, we tried to introduce a cloned Ac element into tobacco cells by an Agrobacterium tumefaciens binary vector system. Transformation of N. babacum cv. Burley 21 tissues and regeneration to whole plant were carried out. The frequency of the transformed callus induced in shoot induction media was higher than that of transformed callus induced in callus induction media. However, the calli were not grown in the second selection media, and became yellow senescent calli. Regenerated tobacco plantlets with foreign gene were also obtained in shoot induction media containing 100
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