Abstract
Carotenoid pigments from muscle of mussel, Mytilus coruscus, and blue mussel edulis, were separated by thin layer and column chromatography. The isolated carotenoids were identified by comparative test with reference carotenoids, reduction with sodium borohydride, isomerization with iodine and absorption spectrophotometry. The carotenoid content in the muscle of mussel were 0.4mg% in male and 2.7mg% in female, and the carotenoids were composed of 23.4%, 33.4% mytiloxanthin, 26.3%, 22.5% 3, 4, 3'-trihydroxy-7', $8'-didehydro-{\beta}-carotene$, 24.8%, 22.8% pectenoxanthin, 14.0%, 9.9% pectenolone and 5.1%, 6.1% diatoxanthin in male and female, respectively. While, the carotenoid contents in the muscle of blue mussel were 1.1mg% in male and 3.2mg% in female, and the carotenoids were composed of 33.8%, 35.6% mytiloxanthin, 28.4%, 44.7% pectenoxanthin, 18.1%, 5.0% diatoxanthin, 9.7%, 8.7% pectenolone and 5.5%, 3.1%, 3, 4, 3'-trihydroxy-7', $8'-didehydro-{\beta}-carotene$ in male and female, respectively.
홍합과 진주담치 근육의 carotenoid를 TLC, column chromatography로 분리하고, $NaBH_4$에 의한 환원반응, $I_2$에 의한 이성화반응 및 UV-visible spectro-photometer로 비교하여 다음과 같은 결론을 얻었다. 혼합 근육의 총 carotenoid함량은 수컷이 0.4mg%, 암컷은 2.7mg%이며, 주 cartenoid는 수컷이 3, 4, 3'-trihydroxy-7', $8'-didehydro-{\beta}-carotene$ 26.3%, pectenoxanthin 24.8% 및 mytiloxanthin 23.4%였고, 그외 pectenolone 14.0%, diatoxanthin 5.1%의 순으로 함유하며, 암컷은 mytiloxanthin 33.4%, pecteno-xanthin 22.8% 및 3, 4, 3'-trihydroxy-7', $8'-didehydro-{\beta}-carotene$ 22.5%였고, 그외 pectenolone 9.9%, diatoxanthin 6.1%의 순으로 함유하여 서로 유사하였다. 진주담치 근육의 총 carotenoid함량은 수컷이 1.1mg%, 암컷은 3.2mg%이며, 주 carotenoid는 수컷이 mytiloxanthin 33.8%, pectenoxanthin 28.4%였고, 그외 diatoxanthin 18.1%, pectenolone 9.7%, 3, 4, 3'-trihydroxy-7', $8'-didehydro-{\beta}-carotene$ 5.5%의 순으로 함유하며, 암컷은 pectenolone 8.7%, diatoxanthin 5.0% 및 3, 4, 3'-trihydroxy-7', $8'-didehydro-{\beta}-carotene$ 3.1%의 순으로 함유하여 서로 유사하였다. 그러나 홍합과 진주담치의 근육 carotenoid는 암컷이 수컷보다 총 carotenoid 함량이 높은 함량치를 보였고, diatoxanthin과 3, 4, 3'-trihydroxy-7', $8'-didehydro-{\beta}-carotene$의 함량에서 차이를 보였다.
