한국미생물·생명공학회지 (Microbiology and Biotechnology Letters)
- 제19권1호
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- Pages.94-99
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- 1991
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- 1598-642X(pISSN)
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- 2234-7305(eISSN)
Methylobacterium sp. GL-10이 생산하는 3-Hydroxybutyrate와 3-Hydroxyvalerate의 Copolyester
Copolyester of 3-Hydroxybutyrate and 3-Hydroxyvalerate Produced by Methylobacterium sp. GL-10
초록
- The further study for the identification of the previously reported pink-pigmented facultative methylotrophic bacterium (PPFM) GL-10 was carried out. The PPFM GL-10 was Gram nagative, rod, and motile by a single polarly inserted flagellum. The colonies were smooth, pink, circular, along with convex with entire margin. The isolate could utilize C1 compounds and a variety of multicarbon substrates as sole carbon and energy source. The isolate was obligately aerobic, and exhibited both catalase and oxidase activities. The deoxyribonucleic acid base composition was 65-67 mol% guanine plus cytosine. The isolate was mostly identical with Methylobacterium extorquens and named Methylobacterium sp. strain GL-10. Methylobacterium GL-10 accumulated a copolyester of 3-hydroxybutyrate and 3-hydroxyvalerate (poly-3HB/3 HV) when grown in nitrogen-free culture media containing sodium propionate as substrate at the second polyester accumulation stage. The composition of copolyester, as determined from