한국미생물·생명공학회지 (Microbiology and Biotechnology Letters)
- 제19권1호
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- Pages.70-75
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- 1991
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- 1598-642X(pISSN)
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- 2234-7305(eISSN)
투과화된 Zymomonas mobilis 균체를 이용한 Glucose와 Fructose로 부터 Gluconic Acid와 Sorbitol의 생산
Continuous Production of Gluconic Acid and Sorbitol from Glucose and Fructose using Perrneabilized cells of Zymomonas mobilis
초록
Continuous and simultaneous production of gluconic acid and sorbitol from glucose and fructose was carried out by using glucose-fructose oxidoreductase and glucanolactonase of Zymomonas mobilis. In order to utilize the enzymes without purification, Zymomonas mobilis was permeabilized with toluene. Optimum conditions for permeabilization and reaction kinetics of permeabilized Zymomonas mobilis were studied. In batch operation with the permeabilized cells immobilized in alginate beads, about 90% conversion was obtained within 35 h reaction. Continuous production of gluconic acid and sorbitol using the immobilized permeabilized cells was carried out. Optimum conditions for continuous operation with the imn~obilized cells were; pH 6.2 and temperature