Microbiology and Biotechnology Letters (한국미생물·생명공학회지)
- Volume 19 Issue 1
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- Pages.52-56
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- 1991
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- 1598-642X(pISSN)
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- 2234-7305(eISSN)
Hydrolysis of Inulin by Endo- and Exo-Inulinase
Endo- 및 Exo-Inulinase를 이용한 Inulin 가수분해
Abstract
Inulin degradation was examined using patially puriiied enzyme mixtures of the Exo-inulinase from a Bacillus spp. and the Endo-inulinase from a Pseudomonas spp.. The highest synergistic xtion of the two cnzymcs was observcd when the Exo- and the Endo-inulinase werc mixed at the ratio of 1 to 13, and the rate of hydrolysis of the above process was enhanced approximately 1.6 times I1ight.1- than that of the reaction catalysed with a single enzyme of the same units. The enzymc mixture showed the maximal activity at pH 6.0 and
부분 정제한 Pseudomonas spp. Endo-inulinase와 Bacillus spp. Exo-inulinase를 13:1의 비율로 혼합 사용하여 inulin을 가수분해한 결과, 단독으로 사용했을 때에 비해 약 1.6배의 현저한 가수분해율의 상승효과를 얻었다. 혼합효소는 반응온도