Extracellular Production of Alpha-Interferon by Recombinant Escherichia coli: PartIV. Effects of Ampicillin and an Inducer on the Production of Alpha-Interferon and Plasmid Stability

유전자 재조합 대장균을 사용한 Alpha-interferon의 생산과 분비;제4부. Ampicillin 및 Inducer의 Alpha-interferon의 생산과 Plasmid 안정성에 미치는 영향

We studied the production and excretion of alpha-interferon in recombinant Escherichia coli harboring plasmid pIF-III-B, which carries alpha-interferon gene under the control of lipoprotein and lacUV5 promoter, and lac operator. Basically, the effects of concentrations of ampicillin and an inducer, IPTG, for the expression of the cloned gene, on the productions of alpha-interferon and plasmid stability were studied. The highest production of alpha-interferon was observed at 50 mg/1 of ampicillin concentration and 0.5 mM of IPTG. The plasmid pIF-III-B was maintained very stably in medium with ampicillin but segregated rapidly in medium without ampicillin. Also, the plasmid was segregated more rapidly in medium with an inducer higher than 0.5 mM.