Changes in the Activities of Isocitrate Dehydrogenase and Glutamate Dehydrogenase and in Free Amino Acid Pool by Heat Shock in Saccharomyces cerevisiae

열자극에 따른 효모 ( Saccharomyces cerevisiae ) 의 Isocitrate Dehydrogenase 와 Glutamate Dehydrogenase 의 활성도 및 유리 아미노산의 변화

Changes in the activities of isocitrate dehydrogenase (IDH) and glutamate dehydrogenase (GDH) and changes in free amino acids in the cytoplasm of Saccharomyces cerevisiae have been studied under heat shock condition. Heat shock conditions led to a significant decrease of NAD-IDH and NAD-GDH, It was shown appeared that the meaningful patterns of increase of NADP-IDH and NADP-GDH. It suggested that heat shock in yeast leads to a splitting of the TCA cycle and that glutamate synthesis takes place through the coupling of the NADP-linked isocirate and glutamate dehydrogenase. It was shown that about 14% of total free amino acids of yeast cells was decreased by heat shock. Especially heat shock condition resulted in the marked decreases of serine family amino acids such as serine, glycine and cysteine, and in the considerable increases of the rates of methionine, alanine, glutamin.