Korean Journal of Microbiology (미생물학회지)
- Volume 29 Issue 5
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- Pages.267-269
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- 1991
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- 0440-2413(pISSN)
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- 2383-9902(eISSN)
Secretion of Membrane-Associated Laccase in Liquid Culture of Coprinus congregatus
Coprinus congregatus의 세포막 연관 laccase의 세포외 분비
Abstract
The hyphal tip laccase of Coprinus congregatus which is a membrane-associated enzyme and shows diffdrdnt banding patterns of PAGE analysis when compared with the enzyme of liquid culture (Choi et al. 1987) has been successfully secreted to culture medium in liquid shake culture by lowering the pH of medium to 4.0. When the fungus is cultivated in YpSs(pH 4.0) liquid, only the hyphal tip laccase is found in the medium after 6 hr incubation and there is no liquid-type enzyme when examined by PAGE analysis.