Korean Journal of Microbiology (미생물학회지)
- Volume 29 Issue 3
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- Pages.172-178
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- 1991
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- 0440-2413(pISSN)
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- 2383-9902(eISSN)
Partial Purification and Characterization of Purine Nucleoside Phosphorylase in Saccharomyces cerevisiae
Saccharomyces cerevisiae에서 PNP의 부분 정제와 특성
Abstract
Intracellular purine nucleoside phosphorylase (PNP) from Saccharomyces cerevisiae was partially purified using ammonium sulfate fractionation, heat treatment, a DEAE-Sephadex A-50 anion exchange chromatography and a Sephadex G-100 gel filtration chromatography. The enzyme was purified 20 fold with 3% recovery. The stability of enzyme was kept by addition of inosine and dithiothreitol. The pH optimum was found to be from 6.3 to 7.3 PNP was sensitive to 10mM of