미생물학회지 (Korean Journal of Microbiology)
- 제29권1호
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- Pages.49-55
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- 1991
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- 0440-2413(pISSN)
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- 2383-9902(eISSN)
Campylobacter jejuni에서 고온충격 단백질의 합성과 내열성
Synthesis and thermotolerance of heat shock proteins in campylobacter jejuni
초록
The heat shock responses of Campylobacter jejuni were studied by examination of their survival rates and synthesis of heat shocd proteins. When C. jejuni cells were treated at the sublethal temperatures of 48.deg.C for 30 minutes, most of the cells maintained their viabilities and synthesized the heat shock proteins of 90, 73, and 66 kD in molecular weight. By the method of two-dimensional electrophoresis, the heat shock proteins of C. jejuni were identified to be Hsp90, Hsp73, and Hsp66. During the heat shock at 48.deg.C, the heat shock proteins were induced from about 5 minutes after the heat shock treatment. Their synthesis was continued upto 30 minutes, but remarkably retarded after 50 minutes. When C. jejune cells were heat shocked at 51.deg.C for 30 minutes, the survival rates of the cells were decreased by about