Korean Journal of Microbiology (미생물학회지)

The Microbiological Society of Korea (한국미생물학회)
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Cloning and Expression of Human Immunodeficiency Virus-1 Epitopes in Escherichia coli

대장균에서 사람의 면역결핍 바이러스-1 epitopes 클로닝과 발현에 대한 연구

  • 유향숙 (한국과학기술연구원 유전공학연구소 분자유전학실) ;
  • 장원희 (한국과학기술연구원 유전공학연구소 분자유전학실) ;
  • 박희동 (한국과학기술연구원 유전공학연구소 분자유전학실) ;
  • 현상원 (한국과학기술연구원 유전공학연구소 분자유전학실) ;
  • 남상욱 (한국과학기술연구원 유전공학연구소 분자유전학실) ;
  • 이영익 (한국과학기술연구원 유전공학연구소 분자유전학실)
  • Published : 1991.03.01
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Abstract

Human immunodeficiency virus type 1 (HIV-1) causes a deadly infectious disease, Acquired Immunodeficiency Syndrome (ADIS). As a first step to develop a reliable and fast diagnostic procedure for HIV-1 infection, we cloned various immunodominant epitopes of HIV-1 in bacterial expression vectors containing tac or trp promoter. While the protein level of direct expression of gp160 was low, trp E fused gp120, gp41 and p17-p24 were produced at high levels (15-30% of total bacterial proteins) in E. coli. Since gp120 and gp41 contain relatively conserved regions which can react with antibodies in the plasma from most of HIV-1 infected individuals, these expression clones were used for large preparations of HIV-1 antigens.

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