Toxicological Research

  • 제7권1호
  • /
  • Pages.1-12
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  • 1991
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  • 1976-8257(pISSN)
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  • 2234-2753(eISSN)
한국독성학회 (Korean Society of Toxicology & Korea Environmental Mutagen Society)
권호 표지

PHENOTYPING OF PHENYTOIN TREATED RAT LIVER CYTOCHROME P-450 WITH MONOCLONAL ANTIBODIES

  • Jin, Hak-Song (College of Phamacy Seoul National University) ;
  • Won, Sun-Me (College of Phamacy Seoul National University) ;
  • Park, Sang-Shin (Laboratory of Molecular Carcinogensis National Cencer Institute National Institute of Health) ;
  • Kim, Nak-Doo (College of Phamacy Seoul National University)
  • 발행 : 1991.06.01
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초록

The phenotyping of cytochrome P-450 in hepatic mivrosomes induced by phenytoin in the rats was carried out by using several monoclonal antibodies (MAbs) against specific P-450 isozymes. Phenytoin (180 mg/kg) was administered intrapritoneally for three consecutive days to the male Sprague-Dawley rats(100-120g). Solid phase radio-immunoassay showed higher binding affinity of MAb PB 2-66-3 and PCN 2-13-1 to the microsomes from phenytoin treated rats than those to from untreated rats, which was comparable to the level in phenobarbital induced rat hepatic microsomes.

키워드

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