The Journal of Korean Physical Therapy
- Volume 3 Issue 1
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- Pages.229-250
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- 1991
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- 1229-0475(pISSN)
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- 2287-156X(eISSN)
The Principles and Practices of Immunocytochemical method in Light Microscopic Level
광학 현미경적 수준에서의 면역조직화학적 방법의 원리 및 실제
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Kim Jin-Sang
(Department of Physical Therapy College of Rehabilitation Science Taegu University)
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김진상
(대구대학교 재활과학대학 물리치료학과)
- Published : 1991.08.01
Abstract
The study was carried out to investigate and review the principles and practices of immunocytochemical method in light microscopic level. The results were as follows. 1. Immunocytochemistry is the method to search out the intracellular position of the specific meterials using antigen -antibody reaction. 2. The chief items in immunocytochemistry are antigen, antibody and chromogen. 3. The identifical fixation is cardiac perfusion fixation. 4. The tissue slides must be prepared by vibratomy. 5. All stainings are carried out with free floating staining method. 6. There are polyclonal and monoclonal antibodies used in immunocytochemistry.