Chemical Mutagenesis of Ulva Pertusa Kjellman by Ethylmethanesulphonate

Ethylmethanesulphonate에 의한 구멍갈파래(Ulva Pertusa Kjellman)의 돌연변이유기

Sensitivity to ethylmethanesulphonate(EMS) on Ulva Pertusa Kjellman thalli was investigated as preliminary studies aimed at mutation breeding of seaweeds. When the thalli were grown in medium containing the mutagen no growth and about $20{\%}$ growth inhibition occurred at $0.05{\%}$ and $0.025{\%}$ EMS-mixed medium respectively. In the case the thalli were treated short time at high concentration mutagen solution followed by culture in control medium no growth and about $10{\%}$ growth inhibition occurred at 40 min. in $1.0{\%}$ and 80 min. in $0.5{\%}$ EMS solution respectively. Among the mutagenized tissues, some showed variation in growth from and cell color contrast to the mother tissue. The mutagenized tissue polypeptide pattern using sodium dodecyl sulphate-polyacrylamide gel electrophoresis was simiiar that of the mother tissue except about 6.6 kD polypeptides.

돌연변이유기를 통한 유용견전형질의 해조류품종을 개발하기 위해 화학적돌연변이유발원인 Ethyl-methanesulphonate(EMS)에 대한 구멍갈파래(Ulva pertusa Kjellman) 엽체의 감수성을 검토하여 효과적인 돌연변이유기조건을 찾고자 하였다. EMS를 처리한 배지에 엽체를 생장시키며 변이를 유발시키고자 하였을 때, $0.05{\%}$와 $0.025{\%}$의 EMS 처리구에서 각각 $100{\%}$와 약 $20{\%}$의 생장억제가 일어났다. 한편 고농도의 EMS액에 엽체를 일정시간 침적한 후 정상배양함으로서 변이를 유발코자 했을 때, $1.0{\%}$ EMS액에 40분과 $0.5{\%}$ EMS액에 80분 처리한 경우 각각 $100{\%}$와 약 $10{\%}$의 생장억제가 일어났다. EMS 처리된 엽체는 생장형과 체색이 모체와 전혀 다른 변이체로 나타나기도 하였는데 이들의 polypeptide 일부에 변화가 있음이 SDS-PAGE로 조사되었다.