Applied Biological Chemistry
- 제34권2호
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- Pages.86-93
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- 1991
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- 2468-0834(pISSN)
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- 2468-0842(eISSN)
cis-Diamminedichloroplatinum(II) (CDDP)에 의한 불루텅 바이러스 이중가닥 RNA의 구조변화
CDDP induces conformational changes in BTV ds RNA rather than forming protein-protein and/or protein-RNA crosslink
- 양재명 (성균관대학교 유전공학과)
- Yang, Jai-Myung (Department of Genetic Engineering, Sung Kyun Kwan University)
- 발행 : 1991.06.30
초록
CDDP는
cis-Diamminedichloroplatinum(II)(CDDP), an antitumor drug, did not generate crosslink between bluetongue virus (BTV) capsid protein at moderate concentration. Cesium chloride density gradient centrifugation study revealed that protein-RNA crosslink was not detectable in CDDP treated BTV. CDDP treated BTV ds RNA showed remarkable change in the migration pattern in polyacrylamide gel electrophoresis. These results suggest that the reduction of BTV core associated transcriptase activity is most likely by the CDDP adduction to the genomic ds RNA rather than by the protein-RNA crosslink and/or protein-protein cross-link.