Antioxygenic Effects of Browning Reaction Product Obtained from L-Ascorbic Acid Solution

L-Ascorbic acid 가열갈변물질의 항산화성

Antioxygenic effects of browning reaction product(BRP) obtained from 2M L-ascorbic acid(AsA) solution by heating at $85^{\circ}C$ were investigated. BRP obtained from AsA solution(pH 2.30) without pH adjusting showed slightly antioxygenic effect. As heating time increased, powers of antioxygenic activity of BRP did not increase. Retained AsA after heating did not effect antioxygenic activity of AsA solution. After adjusting pH of AsA solution to 2.3, 4.0, 7.0, 9.0 and 11.5 respectively, BRP were obtained from these AsA solution by heating at $85^{\circ}C$ for 15 hrs. Among these BRPs, BRPs of pH 2.3 and 4.0 showed no antioxygenic effect, lower browning degree and higher retained AsA, but had stronger reducing power. While those of pH 7.0, 9.0 and 11.5 had stronger antioxygenic activity, higher browing degree and lower retained AsA, but showed weaker reducing power. After adjusting pH of AsA solution to 7.0, antioxygenic activities of BRP which was obtained from this AsA solution by heating respectively at $85^{\circ}C$ for 0, 3, 5, 10, 15 and 25 hrs increased in proportion to heating time.

2M의 AsA용액을 $85^{\circ}C$에서 일정시간 갈변반응시켜서 생성된 갈변물질의 항산화성을 조사하였다. pH를 조절치 않고 가열한 AsA용액(pH2.30)의 갈변반응물질은 AsA를 첨가치 않은 것에 비해 약간의 항산화 효과를 나타내었고 가열시간에 따라서 항산화력의 차이는 거의 나타나지 않았으며, AsA의 잔존량이 항산화력의 세기에 영향을 미치지 않았다. 2M의 AsA용액의 pH를 2.3, 4.0, 7.0, 9.0 및 11.5로 조절한 뒤 $85^{\circ}C$에서 15시간 가열시켜 생성된 갈변물질 중에서 pH2.3, 4.0의 경우는 항산화 효과가 거의 나타나지 않았으며 환원력과 AsA 잔존율은 높았으나 갈변도는 낮았다. 반면 pH7.0, 9.0 및 11.5의 경우는 매우 강한 항산화성을 가지고 있었으며, 이 AsA 갈변용액은 환원력과 AsA 잔존율은 낮았으나 갈변도는 높았다. AsA용액을 pH7.0으로 조절한 후 $85^{\circ}C$에서 0, 3, 5, 10, 15 및 25시간 동안 갈변시켜 얻은 갈변물질의 항산화성은 가열시간이 증가할수록 증가하였다.